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Rat brain membrane-bound delta opioid receptor: Loss and reactivation of binding on dialysis and aging at low temperature

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Title Rat brain membrane-bound delta opioid receptor: Loss and reactivation of binding on dialysis and aging at low temperature
 
Creator Christadoss, E
Oommen, A
 
Description 166-169
A change in the environment
of rat brain membranes by dialysis from phosphate buffered saline (PBS) to 10 mM potassium phosphate (pH 7.2) led to a 35% loss in delta opioid
receptor binding. while alteration of membrane structure on freezing at -20°C
for 55 days led to 85% loss of receptor binding. The dialysate. 200 mM KCI and NaCI restored receptor binding
lost on dialysis. This K+ and Na+ restabilisation of the
receptor can be through cation-π
bonding. interactions that are Suited the lipid bilayer. In membranes stored at
-20°C, the loss or binding is attributed to increased membrane fluidity by phospholipase
A2 action on membrane phospholipids. resulting in an increase of free
fatty acids. K+ but not Na+ restabilization of these
membrane receptors may be due to the ability or K+ 10 decrease
membrane fluidity.
 
Date 2012-12-24T18:40:31Z
2012-12-24T18:40:31Z
2001-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15289
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(3) [June 2001]