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Purification and characterization of a hemagglutinin isolated from the leaves of <i>Chenopodium (Chenopodium amaranticolor)</i>

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Title Purification and characterization of a hemagglutinin isolated from the leaves of Chenopodium (Chenopodium amaranticolor)
 
Creator Suseelan, K N
Mitra, R
 
Description 186-192
A hemagglutinin was isolated and purified
from the leaves of Chenopodium (Chenopodium amaranticolor) using
ionexchange chromatography and affinity chromatography on fetuin- agarose
matrix . It agglutinated rabbit erythrocytes. The

hemagglutinin had a native molecular mass
of 58 kDa, as estimated by gel filtration and showed a single band of molecular
mass of 33 kDa on SDS-PAGE. It showed
hemagglutinati on activity over the pH range 3-12 and was found to be stable up

to 70°C. On isoelectrofocussing, the pl of this hemagglutinin was estimated
to be 5.25. However, it was found to contain seven charge variants when isoelectrofocussing
was performed in presence of 6M urea.
 
Date 2012-12-24T19:12:42Z
2012-12-24T19:12:42Z
2001-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15293
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(3) [June 2001]