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Biochemical effects of sparfloxacin on cell envelope of mycobacteria

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Title Biochemical effects of sparfloxacin on cell envelope of mycobacteria
 
Creator Kaur, Devinder
Verma, Indu
Khuller, G K
 
Description 235-240
Sparfioxacin, a difluorinated quinolone is a potent
anti -mycobacterial agent used in the treatment of mycobacterial infections. We
have investigated whether sparfloxacin had other, more subtle effects on
mycobacteria besides its interaction with DNA gyrase that could contribute to
its therapeutic efficacy. Mycobacterium smegmatis cells grown in media
with subinhibitory

concentration of sparfioxacin were observed to have
significant reduction in the biosynthesis of vital macromolecules, as shown by
the incorporation of various radiolabelled precursors. The analysis of
subcellular distribution

of phospholipids of sparfloxacin-treated cells
demonstrated an increase in the cell membrane and reduction in the cell wall, suggesting
changes in the cell envelope architecture by sparfioxacin. Significant changes
were also obscrved in other chemical constituents of the cell wall, especially
in the arabinose and glucosamine contents. Mycolic acids, the major

component of mycobacterial cell wall were reduced in
the presence of MIC50 of sparfloxacin. There was a decrease in the limiting
fluorescence intensity (Fmax) of  I-anilinonaphthalene 8-sulfonate (ANS)
indicating alterations in the organization and conformation of mycobacterial
cell surface. These results suggest that the mechanism of action of anti-mycobacterial

action of sparfloxacin involves mycobacterial cell
envelope.
 
Date 2012-12-25T18:19:47Z
2012-12-25T18:19:47Z
2001-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15299
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(4) [August 2001]