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Purification of vitellogenin from the plasma of Indian freshwater murrel, <i>Channa punctatus </i>(Bloch) by different methods: A comparative study

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Title Purification of vitellogenin from the plasma of Indian freshwater murrel, Channa punctatus (Bloch) by different methods: A comparative study
 
Creator Sehgal, Neeta
Goswami, SV
 
Description 263-269
Plasma from estrogenized, [32p] NaH2P04 -injected
murrel, Channa
Punctatus was
collected in the
presence of proteolytic inhibitors and subjected to different separation procedures singly or in combination,
viz., gel filtration chromatography on Ultrogel AcA 34, ion-exchange chromatography on DEAE sephacel, or selective precipitation with dimethylformamide or with Mg2+:
EDTA in order to
isolate vitellogenin from other plasma proteins. The results show that chromatography on Ultrogel or DEAE sephacel yields intact vitellogenin whereas prior
precipitation with DMF or with

Mg2+: EDT A results in either co-precipitation of other plasma proteins or in the cleavage of phosvitin-like material from the native vitellogenin molecule.
 
Date 2012-12-25T18:24:52Z
2012-12-25T18:24:52Z
2001-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15304
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(4) [August 2001]