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Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride Membrane

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Title Purification of tryptic peptides for mass spectrometry using polyvinylidene fluoride Membrane
 
Creator Kurien, B T
Matsumoto, H
Scofield, R H
 
Description 274-276
A simple procedure
for the purification of tryptic peptides, prior to mass spectrometric analysis,
using polyvinylidene fluoride membrane (PVDF) is described. The sensitivity of
mass spectrometric analysis is such that minor impurities in tryptic peptide digests
suppress the signal obtained. However, we obtained useful signal, from a sample
that did not yield any spectra earlier, by purifying the sample using PVDF
membrane. For this. the tryptic peptide digest was first spotted on the membrane
which was then air-dried and washed. Further, the membrane was extracted with
trifluoroacetic acid (TFA) and acetonitrile and subjected to mass spectrometric
analysis. This procedure enabled us to identify a cross-reactive D1 antigen on
the neutrophil surface that bound antibodies that targeted 60 kD Ro autoantigen
in systemic lupus erythematosus, an autoimmune disorder.
 
Date 2012-12-25T18:26:41Z
2012-12-25T18:26:41Z
2001-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15306
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(4) [August 2001]