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Role of positive charge of lysine residue on ribosome-inactivating property of gelonin

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Title Role of positive charge of lysine residue on ribosome-inactivating property of gelonin
 
Creator Singh, Ranjit C
Alam, Anis
Singh, Vinod
 
Description 309-312
The report that gelonin cross-linked with monoclonal antibodies with
the use of 2-iminothiolane (2-IT) exhibited higher cytotoxicity than the
conjugates prepared with the use of N-succinimidyl-3-(2-pyridylthio) propionate
(SPDP) alone, has prompted us to investigate the effect of ɛ-NH2
group modification with 2-IT on the ribosome-inactivating property (RIP) of gelonin.
The purified gelonin was modified with 2-IT at a different molar ratio and
their effects on immunoreactivity and ribosome-

inactivating property were compared with those of N-succinimidyl
6-[3-(2-pyridyldithio) propionamido ] hexanoate (long chain-SPDP) and SPDP
modified gelonin derivatives. Modification of single amino group with 2-IT
results in about

25-50% inhibition of immunoreactivity and 60-70%
loss of protein synthesis inhibition activity. Modification of 2-3 amino groups
further hampers both immunoreactivity and protein synthesis inhibition property
of gelonin. Both the long chain-SPDP with SPDP modifications showed more
pronounced effects on immunoreactivity and RIP activity as compared to the similar
ratio of 2-IT modification(s). It may, therefore, be concluded that the positive
charge plays an important role in the immunological as well as the protein
synthesis inhibitory effect of gelonin .
 
Date 2012-12-25T18:44:30Z
2012-12-25T18:44:30Z
2001-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15319
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(5) [October 2001]