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Insulin receptor tyrosine kinase activity in monocytes of type 2 diabetes mellitus patients receiving oral L-lysine

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Title Insulin receptor tyrosine kinase activity in monocytes of type 2 diabetes mellitus patients receiving oral L-lysine
 
Creator Sulochana, K N
Rajesh, M
Ramakrishnan, S
 
Description 331-334
The action of lysine as an antidiabetic agent was examined
in human volunteers. Eight patients with type 2 DM were orally supplemented
with L- lysine hydrochloride lg /day in two doses along with antidiabetic
tablets (glyciphage  or chlorformine), for
a period of two months. Periodically their plasma fasting sugar and insulin receptor
tyrosine kinase activity was measured in their monocytes. Eight normal healthy
volunteers served as controls for comparison of receptor tyrosine kinase activity.
Insulin receptor tyrosine kinase was isolated from monocytes by
immunoprecipitation and the activity was determined using exogenous substrate
poly glu-tyr (4: 1) and radioactive ATP. Phosphorylated peptide was separated
by electrophoresis and quantified using a liquid scintillation system. The
enzyme activity was significantly low (22074±1728 dpm/ml immunoprecipitate) in
subjects with diabetes when compared to non-diabetic control group (50,775 ± 3597).
Lysine treatment enhanced the enzyme activity by 31 % in patients with diabetes and decreased their blood sugar by 27% .
 
Date 2012-12-25T18:37:29Z
2012-12-25T18:37:29Z
2001-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15312
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(5) [October 2001]