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<span style="font-size:14.0pt;font-family:Fd35564-Identity-H;mso-bidi-font-family: Fd35564-Identity-H;color:#090909">Physico-chemical characterization of growth hormone from water buffaloes <span style="font-size:14.0pt;font-family: Fd234404-Identity-H;mso-bidi-font-family:Fd234404-Identity-H;color:#090909">(<i style="mso-bidi-font-style:normal">Bubalus bubalis</i>) </span></span>

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Title Physico-chemical characterization of growth hormone from water buffaloes (Bubalus bubalis)
 
Creator Maithal, Kapil
Krishnamurty, H G
Muralidhar, K
 
Description 375-383
A purified preparation of growth hormone
from pituitaries of water buffaloes (Bubalus bubalis) has been extensively characterized with
regard to physico-chemical properties. The molecular size of buffalo GH (buGH)
by electrospray ionization mass spectroscopy (ES-MS) was found to be 21394.00 ± 8.44Da and its stokes radius was determined as 2.3 nm. Size
heterogeneity in buffalo GH was checked both by electrophoresis and molecular
sieve chromatography using 1251-labelled buffalo GH. Similar size
heterogeneity was found in standard preparations of ovine and bovine growth
hormones.

Isoelectric focussing and chromatofocussing
indicated charge heterogeneity in buffalo GH preparation. Major charge isoforms
having pI of 7.2, 7.7 and minor forms
in the iI range of 5.7 to 7.0 were found. Lectin chromatography on Concanavalin
A matrix showed that less than 1 % of buffalo GH was glycosylated.
Heterogeneity in NH2-terminal sequence was also observed, with
alanine, phenylalanine and methionine as the NH2-terminal residues
as checked by dansyl and DABITC methods. Estimation of tryptophan residue
indicated that a single tryptophan residue was present. Ellman's method showed
presence of two disulfide bridges per mole of buffalo GH. Intrinsic
fluorescence spectrum of buffalo GH exhibited A emission maximum at 337
nm. UV-CD spectrum showed that almost 48% of the secondary structure of buGH was
constituted by α-helicity. The TM of buGH as determined by differential
scanning calorimetric (DSC) studies was found to be 63°C.
 
Date 2012-12-25T18:57:53Z
2012-12-25T18:57:53Z
2001-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15323
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(6) [December 2001]