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Buffalo plasma fibronectin : A physico-chemical study

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Title Buffalo plasma fibronectin : A physico-chemical study
 
Creator Ahmed, Nizamuddin
Chandra, Ramesh
Raj, H G
 
Description 384-392
Plasma fibronectin (FN) of buffalo (Babulis
babulis) was purified to apparent homogeneity, using gelatin-Sepharose and
heparin-Sepharose affinity columns. It was found to have two subunits of
molecular mass 246 kDa and 228 kDa, on SDS-gel. Its immunological cross-reactivity
with anti -human plasma FN was confirmed by Western blotting. The amino acid
composition was found to be similar to that of human and bovine plasma FNs.
Buffalo plasma FN contained 2.23% neutral hexoses and 1.18% sialic acids. No
titrable sulfhydryl group could be detected in the absence of denaturant.
Reaction with DTNB indicated 3.4 sulfhydryl groups in the molecule, whereas
BDC-OH titration gave a value of 3.8 -SH groups in buffalo plasma FN. Stoke's
radius, intrinsic viscosity, diffusion coefficient and frictional ratio
indicated that buffalo plasma FN did not have a compact globular conformation
at physiological pH and ionic
strength. Molecular dimensions (average length, 120 nm; molar mass to length
ratio, 3950 nm-1 and mean diameter, 2.4 nm) as revealed by rotary
shadowing electron microscopy further supported the extended conformation of
buffalo plasma FN. These results show that buffalo plasma FN has similar
properties as that of human plasma FN.
 
Date 2012-12-25T18:59:57Z
2012-12-25T18:59:57Z
2001-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15324
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(6) [December 2001]