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Effect of a water soluble derivative of α-tocopherol on radiation response of <i>Saccharomyces cerevisiae</i>

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Title Effect of a water soluble derivative of α-tocopherol on radiation response of Saccharomyces cerevisiae
 
Creator Singh, Rakesh K
Verma, Naresh C
Kagiya, V T
 
Description 399-405
The radioprotection conferred by a highly water soluble
glucose derivative of α -tocopherol, namely, 2-( α-D-glucopyranosyl) methyl-2.5,7,8-tetramethylchroman-6-ol
(TMG) in Saccharomyces cerevisiae was studied. Cells grown in standard
YEPD-agar medium and irradiated in the presence of TMG showed a concentration
dependent higher survival up to 10 mM of TMG in comparison to cells
irradiated in distilled water. Treatment of TMG to cells given either before or
immediately after irradiation but not during irradiation, had no effect on
their radiation response. S.cerevisiae strain LP1383 (rad52) which
is defective in recombination repair showed enhanced radioresistance only when
subjected to irradiation in

presence of TMG. Cells of radS2 strain grown in
the medium containing TMG showed a radiation response similar to that of cells
grown in the medium without TMG. The nature of TMG dependent enhanced radioresistance
was studied by scoring the mutations in the strain D-7, which behaved like wild
type strain in complete medium, at trp and ilv loci . Our study
indicated that TMG confers radioresistance in S. cerevisiae possibly by
two mechanisms viz. (i), by eliminating radiation induced reactive free radicals
when the irradiation is carried out in the presence of TMG and (ii ), by activating
an error - prone repair process involving RAD52 gene, when the cells are
grown in the medium containing TMG.
 
Date 2012-12-25T19:01:25Z
2012-12-25T19:01:25Z
2001-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15326
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(6) [December 2001]