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Effect of γ-irradiation on H3 histone and DNA in solution

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Title Effect of γ-irradiation on H3 histone and DNA in solution
 
Creator Upadhyay, S N
 
Description 406-411
Three methods, namely, absorbance of colour
by reaction with Folin-Ciocalteau reagent, UV absorbance and fluorescence
intensity measurements for detection of H3 histone in 0.15 M standard
saline citrate (SSC) solution were compared. Maximum sensitivity was found with
the Folin-Ciocalteau method. Effect of varying pH and of γ - radiation on H3 hi
stone and on interaction of H3 hi stone with DNA were studied. For this,
solutions of H3 histone in SSe. in 0.9% NaCl, H3 hi stone + DNA in 0.9% NaCI were subjected to varying pH (1-10) and γ - radiation (dose 10-50 Gy) and λmax and Aλmax),max
were monitored. From the molar ratios of histone and DNA in the complex, it was
observed that at γ-radiation dose of 50 Gy and pH 8.54, there was a depletion of 6-8 μg/ml of histone from the
histone-DNA complex .
 
Date 2012-12-25T19:02:32Z
2012-12-25T19:02:32Z
2001-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15327
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(6) [December 2001]