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Urate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determination
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Urate oxidase electrode based on dissolved oxygen probe for urine uric acid, Determination
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| Creator |
Dinckaya, Erhan
Akyilmaz, Erol Akgol, Sinan |
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| Description |
67-70
A biosensor for the specific determination of uric acid in urine was developed using urate oxidase (EC 1.7.3.3) in combination with a dissolved oxygen probe. Urate oxidase was immobilized with gelatin by means of glutaraldehyde and fixed on a pretreated teflon membrane to serve as enzyme electrode. The electrode response was maximum when 50 mM glycine buffer was used at pH 9.2 and 35°C. The enzyme electrode response depends linearly on uric acid concentration between 5- 40 μM with a response time of 5 min. The enzyme electrode is stable for more than 2 weeks and during this period over 35 assays were performed. |
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| Date |
2012-12-28T11:33:52Z
2012-12-28T11:33:52Z 2000-02 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15373 |
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| Language |
en_US
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| Rights |
 CC Attribution-Noncommercial-No Derivative Works 2.5 India
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBB Vol.37(1) [February 2000]
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