Record Details

Ribosome-inactivating property of gelonin is more affected by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido]hexanoate modification than N-succinimidyl-3- (2-pyridylthio) propionate

NOPR - NISCAIR Online Periodicals Repository

View Archive Info
 
 
Field Value
 
Title Ribosome-inactivating property of gelonin is more affected by N-succinimidyl 6-[3-(2-pyridyldithio) propionamido]hexanoate modification than N-succinimidyl-3- (2-pyridylthio) propionate
 
Creator Singh, Vinod
Singh, Ranjit C
Dubey, Rajesh Kumar
Alam, Anis
 
Description 155-165
As gelonin cross-linked to carrier proteins has been
found as an effective hybrid complex for selective targeting to specific cells,
the ε-NH2 group of gelonin(s)
obtained by different methods has been sequentially modified by N-succinimidyl 6-[3-(2-pyridyldithio)
propionamido] hexanoate (long chain-SPDP) and its effect on immunoreactivity
and ribosome inactivating

property has been compared with that of
N-succinimidyl-3-(2-pyridylthio) propionate (SPDP) modified gelonin. Modification
of single amino group results in about 80% inhibition of immunoreactivity and
more than 90% loss of protein synthesis-inhibition activity. Modification of
2-3 amino groups further hampers both immunoreactivity and protein synthesis inhibition
property of gelonin. Upon comparison of long chain-SPDP with SPDP modification,
the long chain-SPDP modification plays more pronounced effect on
immunoreactivity and ribosome-inactivating property (RIP) activity than that of
SPDP. It may, therefore, be concluded that the increase in carbon-chain spacer
arm, although may provide less steric hindrance for receptor recognition of the
carrier protein, has inhibitory effect on the cytotoxic activity of gelonin .
 
Date 2012-12-28T19:47:37Z
2012-12-28T19:47:37Z
2000-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15381
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(3) [June 2000]