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Age dependent alterations in photosystem II acceptor side in <i>Cucumis sativus</i> cotyledonary leaf thylakoids: Analysis of binding characteristics of herbicide <span style="font-size:12.0pt;font-family:"Times New Roman"; mso-fareast-font-family:"Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language: EN-IN;mso-bidi-language:AR-SA" lang="EN-IN">[<sup>14</sup>C]-atrazine</span>

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Title Age dependent alterations in photosystem II acceptor side in Cucumis sativus cotyledonary leaf thylakoids: Analysis of binding characteristics of herbicide [14C]-atrazine
 
Creator Prakash, J S S
Baig, M A
Mohanty, Prasanna
 
Description 10-13
Senescence induced
temporal changes in photosystems can be conveniently studied in cotyledonary
leaves. We monitored the protein, chlorophyll and electron transport activities
in Cucumis sativus cv Poinsette cotyledonary leaves and observed that by
20th day, there was a 50%, 41 % and 30-33% decline in the chlorophyll, protein
and photosystem II activity respectively when compared to 6th day cotyledonary
leaves taken as control. We investigated the changes in photosystem II activity
(O2 evolution) as a function of light intensity. The
photosystem II functional antenna decreased by 27% and the functional
photosystem II units decreased by 30% in 20-day old cotyledonary leaf
thylakoids. The herbicide [14C]-atrazine binding assay to monitor
specific binding of the herbicide to the acceptor side of photosystem II
reaction centre protein, D I, showed an increase in the affinity for atrazine
towards D1 protein and decrease in the QB binding sites in 20th day leaf thylakoids
when compared to 6th day leaf thylakoids. The western blot analysis also
suggested a decrease in steady state levels of D1 protein in 20th day
cotyledonary leaf thylakoids as compared to 6th day sample which is in
agreement with [14C]-atrazine binding assay and light saturation
kinetics.
 
Date 2012-12-30T20:24:19Z
2012-12-30T20:24:19Z
1999-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15410
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(1) [February 1999]