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Chemical characterization of t<span style="font-size:14.0pt;font-family:HiddenHorzOCR;mso-bidi-font-family:HiddenHorzOCR">he lipopolysaccharides from enteropathogenic <i>Escherichia coli </i>O142 and O158 </span>

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Title Chemical characterization of the lipopolysaccharides from enteropathogenic Escherichia coli O142 and O158
 
Creator Datta, Anup Kr
Basu, Sumanta
 
Description 55-58
Lipopolysaccharides (LPS) from two enteropathogenic
strains of E. coli O142 and O158 were isolated by hot phenol-water
extraction procedure. Polyacrylamide gel electrophoretic pattern of the LPS showed
the typical ladder like pattern of smooth type of LPS. The LPS of E. coli O158
was  found to contain L-rhamnose, D-glucose
and N-acetyl-D-galactosamine as major constituents together with D-galactose, N-acetyl-D-glucosamine,
L-glycero-D-manno-heptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO)
whereas LPS from E. coli O142 contained L-rhamnose,
N-acetyl-o-glucosamine and N-acetyl-o-galactosamine as major constituents
together with D-glucose, D-galactose, N-acetyl-D-glucosamine,
L-glycero-D-mannoheptose and 2-keto-3-deoxy-D-manno-octulosonic acid (KDO). LPS

was degraded by mild acid hydrolysis to yield a
degraded polysaccharide fraction and an insoluble lipid-A fraction. The main fatty
acids of the lipid-A fraction of the LPS were C12:0, C14:0, and 3-OH C14:O for O158
strain whereas E. coli O142
lipid-A consisted of C12:0, C14:0, 3-OH C14:0, and C16:0 . The degraded
polysaccharide fraction on gel permeation  chromatography gave a high, moleculer weight
O-chain fraction and a core

oligosaccharide and a fraction containing degraded
sugars. The chemical composition of LPS and its fragmented products are reported
in this communication .
 
Date 2012-12-30T20:23:53Z
2012-12-30T20:23:53Z
1999-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15409
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(1) [February 1999]