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<i style="mso-bidi-font-style:normal"><span style="font-size:12.0pt;font-family:"Times New Roman";mso-fareast-font-family: "Times New Roman";mso-ansi-language:EN-IN;mso-fareast-language:EN-IN; mso-bidi-language:AR-SA" lang="EN-IN">n</span></i><span style="font-size:12.0pt; font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:AR-SA" lang="EN-IN">-Propanol as a substrate for assaying the ligninperoxidase activity of <i>Phanerochaete chrysoporium</i></span>

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Title n-Propanol as a substrate for assaying the ligninperoxidase activity of Phanerochaete chrysoporium
 
Creator Shanmugam, V
Kumari, Meera
Yadav, K D S
 
Description 39-43
The steady state
kinetics of ligninperoxidase catalysed reaction using n-propanol as the organic substrate and monitoring the formation of
propanaldehyde at λ = 300 nm spectropbotometerically as functions of different
reaction parameters has been studied. It has been concluded that n-propanol can be used as a substrate
for analysing the activity of ligninperoxidase. The turnover number of ligninperoxidase of Phanerochaete chrysosporium using n-propanol as substrate has been found
to be higher approximately by a factor of 103 as compared to that
using veratryl alcohol as the substrate. The method works in assaying the
activity of Iigninperoxidase produced by Aspergillus fumigatus indicating
that it can be used for assaying the ligninperoxidase activities produced by
other microorganisms also and is not limited to assaying the ligninase activity
produced by Phanerochaete chrysosporium alone. Under identical experimental
conditions, horseradish peroxidase does not show peroxidase activity using n-propanol as substrate indicating that
the method does not interfere with the activities of other peroxidases.
 
Date 2012-12-30T20:24:49Z
2012-12-30T20:24:49Z
1999-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15411
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(1) [February 1999]