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Interaction of chlorpromazine with low molecular mass ion-transporting ATPase modulator proteins from rat brain cytosol

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Title Interaction of chlorpromazine with low molecular mass ion-transporting ATPase modulator proteins from rat brain cytosol
 
Creator Bhattacharyya, Dipankar
Sen, Parimal C
 
Description 82-87
Two low molecular mass proteins (13 kDa which inhibits Na+,
K+-ATPase and 12 kDa which modulates Ca2+, Mg2+- and Ca2+-ATPases),
purified from rat brain cytosol form complexes with chlorpromazine (CPZ) on
incubation. The conformational characteristics of the proteins and their
complex have been studied by comparing the fluorescence and CD spectra. The
tryptophan fluorescence data show that the inhibitor-CPZ complex does not
quench the fluorescence of Na+, K+ -ATPase significantly.
CD spectra indicate that the structure of the inhibitor is changed on format
ion of the complex. The inhibitor-CPZ complex significantly changes the
conformation of Na+, K+-ATPase. The regulator protein-CPZ
complex does not have any appreciable effect on Ca2+, Mg2+ - and Ca2+-ATPase activities.
The Trp-fluorescence of Ca2+, Mg2+-and Ca2+-ATPases are not significantly affected in presence of the complex.
CD spectra indicate that the structure of the regulator is abruptly affected on
formation of the complex. The conformations of Ca2+, Mg2+-and
Ca2+-ATPases are found to be altered in presence of the complex.
 
Date 2012-12-31T19:11:38Z
2012-12-31T19:11:38Z
1999-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15432
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(2) [April 1999]