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Two new functions of inositol in the eye lens: Antioxidation and antiglycation and possible mechanisms

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Title Two new functions of inositol in the eye lens: Antioxidation and antiglycation and possible mechanisms
 
Creator Ramakrishnan, S
Sulochana, K N
Punitham, R
 
Description 129-133
The extent of glycation of human eye lens proteins with glucose in
presence of added inositol was examined in vitro using [U 14C] glucose.
Lens homogenate was reacted with varying concentrations of glucose and glucose
+ inositol. At the end of the reaction, the proteins were precipitated with
TCA, centrifuged, dissolved in NaOH and the radioactivity was measured.
Inositol decreased the glycation by 57-67 %.  Pure inositol and glucose suitably labelled
with 3H or 14C when reacted and followed by paper
chromatography and HPLC showed that glucosyl inositol was present along with
unreacted free glucose. Preliminary studies made of the UV spectra of pure
inositol (i) when reacted with H2  O2
showed that inositol removed H2 O2 from the reaction
mixture (ii) when reacted with arachidonic acid showed that they formed a
conjugate. The observations indicate that the antioxidant property of inositol
could be the result of its' quenching action on reactive oxygen, intermediates
and conjugate-formation with compounds like arachidonic acid and the
antiglycating property due to

scavenging of glucose. The antioxidant and the
antiglycating properties of inositol may be beneficial in delaying or averting cataract.
 
Date 2012-12-31T19:05:06Z
2012-12-31T19:05:06Z
1999-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15425
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(2) [April 1999]