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<span style="font-size:12.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-ansi-language: EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:AR-SA" lang="EN-IN">Kinetic mechanism of glucose dehydrogenase from <i>Halobacterium salinarum</i></span>

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Title Kinetic mechanism of glucose dehydrogenase from Halobacterium salinarum
 
Creator Bhaumik, S R
Sonawat, H M
 
Description 143-149
The kinetic mechanism
of glucose dehydrogenase (EC 1.1.1.47) from  Halobacterium salinarum was
studied by initial velocity and product inhibition methods. The results suggest
that both, in the forward and reverse direction, the reaction mechanism is of
Bi Bi sequential ordered type involving formation of ternary complexes. NADP+
adds first and NADPH formed dissociates from the enzyme last. For the reverse
direction, NADPH adds first and NADP+ leaves last. Product
inhibition experiments indicate that (a), the coenzymes compete for the same
site and form of the enzyme and (b), ternary abortive complexes of enzyme-NADP+-glucono-δ-lactone
and enzyme-NADPH-glucose are formed. All the other inhibitions are
noncompetitive.
 
Date 2012-12-31T19:13:31Z
2012-12-31T19:13:31Z
1999-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15433
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(3) [June 1999]