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Binding of cationic surfactants to DNA, protein and DNA-protein mixtures

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Title Binding of cationic surfactants to DNA, protein and DNA-protein mixtures
 
Creator Gani, SA
Chattoraj, D K
Mukherjee, DC
 
Description 165-176
Extent of binding of cationic
surfactants cetyltrimethyl ammonium bromide (CTAB), myristyltrimethyl ammonium
bromide (MTAB) and dodecyl trimethyl ammonium bromide (DTAB) to calf-thymus
DNA, bovine serum albumin (BSA) and to their binary mixture respectively have
been measured as function of bulk concentration of the surfactant by using
equilibrium dialysis technique. Binding of CTAB has been studied at different pH, ionic strength (μ), temperature and
biopolymer composition and with native and denatured states of the biopolymers.
The chain-length of different long chain amines plays a significant role in the
extent of binding under identical solution condition. The binding ratios for CTAB
to collagen, gelatin, DNA-collagen and DNA-gelatin mixtures respectively have
also been determined. The conformational structures of different biopolymers
are observed to play significant role in maqomolecular interactions between
protein and DNA in the presence of CTAB. From the experimental values of the
maximum binding ratio () at the saturation level for each individual biopolymer, ideal
values ()id have been
theoretically calculated for binary mixtures of biopolymers using additivity
rule. The protein-DNA-CTAB interaction in mixture has been explained in terms of
the deviation (∆) of () from ()id in the presence of a surfactant in bulk. The binding of surfactants
to biopolymers and to their binary mixtures are compared more precisely in
terms of the Gibbs' free energy decrease (-∆Go)
for the saturation of the binding sites in the biopolymers or biopolymer mixtures
with the change of the bulk surfactant activity from zero to unity in the
rational mole fraction scale.
 
Date 2012-12-31T19:19:43Z
2012-12-31T19:19:43Z
1999-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15437
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(3) [June 1999]