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Deconvolution of the fluorescence spectra into its component Gaussians: A method to analyze the binding of coumarin to bovine serum albumin

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Title Deconvolution of the fluorescence spectra into its component Gaussians: A method to analyze the binding of coumarin to bovine serum albumin
 
Creator Bharathi, Sivakamasundari
Mishra, A K
 
Description 188-194
7-N,N-Diethylamino-4-methylcoumarin, (cou-I), a readily available
laser dye binding to bovine serum albumin (BSA). at room temperature has been
studied by steady state fluorescence spectroscopy. Existing methods of analysis
of the binding to obtain the binding parameters are based on the change in
fluorescence intensity at a particular wavelength. These methods are not
convenient when there is a gradual shift in the emission maxima for increasing
protein concentration. In this paper we present a method to obtain the binding
constants of cou-I to BSA using a Windows '95 based package to deconvolute the
asymmetrical spectrum (fluorescence intensity versus wave number curve)
into two Gaussians, each corresponding to the binding of the fluorophore to a
particular site. This method is convenient to analyze the binding constant data
and obtain the binding parameters of each binding site, and can also provide
information about the microenvironment of each site, relating micropolarity and
microviscosity.
 
Date 2012-12-31T19:20:23Z
2012-12-31T19:20:23Z
1999-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15438
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(3) [June 1999]