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Solubilization and binding of DNA-CTAB complex with SDS in aqueous media

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Title Solubilization and binding of DNA-CTAB complex with SDS in aqueous media
 
Creator Gani, SA
Chattoraj, D K
Mukherjee, D C
 
Description 233-239
Extent of binding () of sodium dodecyl sulphate (SDS) to the binary complex formed
between calfthymus DNA. and cetyltrimethylammonium bromide (CTAB) has been
measured in mole per mole of nucleotide in the complex as function of
concentration of SDS by using equilibrium dialysis technique at different
temperatures and pH. Binding of SDS to thermally denatured DNA-CTAB
complex has also been studied. The most interesting aspect to be noted in this
experiment is that the water insoluble DNA-CTAB binary complex gets solubilized
in the ternary mixture in presence of SDS but when DNA is thermally denatured,
the ternary system DNA-CTAB-SDS remains insoluble. Significant change in the
extent of binding has been noted with the variation of the relative composition
of DNA and CTAB in their binary mixture. The data of binding of SDS to DNA-CTAB
complex are compared more precisely in terms of the standard Gibbs' free energy
decrease (-∆Go)
for the saturation of the binding sites in the complex with the change of
SDS activity from zero to unity in the rational mole fraction scale.
 
Date 2012-12-31T19:27:52Z
2012-12-31T19:27:52Z
1999-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15446
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(4) [August 1999]