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Physicochemical characterization of hepatic glucocorticoid receptors from pre- and post-weaned mice

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Title Physicochemical characterization of hepatic glucocorticoid receptors from pre- and post-weaned mice
 
Creator Borbhuiya, Monsur A
Sharma, Ramesh
 
Description 240-247
The physicochemical
parameters viz., molecular weight, stokes radius and ionic state of hepatic
glucocorticoid receptors from pre-(10-day) and post-(60-day) weaned mice were
studied. Gel permeation studies of the crude receptors showed a molecular mass
of ~290 kDa for the unactivated receptors from both the age groups while the
thermally activated receptors showed a molecular mass of ~90 kDa. The stokes
radii were ~5.8 and 3.6 for the unactivated and activated receptors,
respectively from both the age groups studied. Elution of the bound
glucocorticoid receptors from anion-exchanger did not reveal any charge
difference in the two age groups; the unactivated receptors eluted at ~250 mM KCl whereas the activated receptors
eluted at ~100 mM KCI.
Salt extraction of thermally activated nuclear bound receptors and
immunological studies on the unactivated receptors revealed no age-related
variation in the two groups of mice. Our findings confirm that the physicochemical
properties of hepatic glucocorticoid receptors remain unchanged at these
developmental stages of mice.
 
Date 2012-12-31T19:30:35Z
2012-12-31T19:30:35Z
1999-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15449
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(4) [August 1999]