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<span style="font-size:12.0pt;font-family: "Times New Roman";mso-fareast-font-family:"Times New Roman";mso-ansi-language: EN-IN;mso-fareast-language:EN-IN;mso-bidi-language:AR-SA" lang="EN-IN">Purification and characterisation of gelonin from seeds of <i>Gelonium multiflorum</i></span>

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Title Purification and characterisation of gelonin from seeds of Gelonium multiflorum
 
Creator Singh, Vinod
Singh, Ranjit C
Dubey, Rajesh Kumar
Alam, Anis
 
Description 258-265
Gelonin, a ribosome-inactivating protein has been isolated from the
seeds of Gelonium multifluorum of Euphorbiaceae family by two methods
and the results are compared. In method-I conventional aqueous extraction, cation-exchange
and gel-filtration chromatography has been used. In method-II S-Sepharose fast now
gel has been used to purify the proteins from the seed extract, followed by
ammonium sulfate fractionation, cation-exchange and gel-filtration
chromatography. Extensive physico-chemical and immunological characterizations
show that molecular weight of gelonin as determined by gel-filtration
chromatography and SDS-PAGE is -30 kDa. The non-proteinous material which binds
to CMC-gel in association with gelonin in method-I is substantially removed
when gelonin is purified
by method-II. Cation exchange, G-100 chromatography,
RP-HPLC and SDS-PAGE show that method-II yields 50% more purified gelonin when compared
to the yield by method- I. The immunoreactivity of gelonin obtained by methods
I and II vary from 22-26% and 50-66%, respectively and the
ribosome-inactivating property vary from 46-56% and 70-87% respectively.
 
Date 2012-12-31T19:29:08Z
2012-12-31T19:29:08Z
1999-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15447
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(4) [August 1999]