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Pathways of glucose catabolism in procyclic <i>Trypanosoma congolense</i>

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Title Pathways of glucose catabolism in procyclic Trypanosoma congolense
 
Creator Obungu, Victor H
Kiaira, Job K
Olembo, Norah K
Njogu, Muturi R
 
Description 305-311
Studies of respiration on glucose in procyclic Trypanosoma
congolense in the presence of rotenone,antimycin, cyanide, salicylhydroxamic
acid and malonate have indicated the presence of NADH dehydrogenase, cytochrome
b-c1, cytochrome aa3, trypanosome alternate
oxidase and NADH fumarate reductase/succinate dehydrogenase pathway that contributes
electrons to coenzyme Q of the respiratory chain. The rotenone sensitive NADH
dehydrogenase, the trypanosome  alternate
oxidase, and cytochrome aa3, accounted for 24.5±6.5, 36.2±4.2
and 54.1±5.5% respectively of the total respiration. Activities of lactate
dehydrogenase, NAD+- linked malic enzyme and pyruvate kinase were
less than 6 nanomoles/min/mg protein suggesting that they play a minor role in
energy metabolism of the parasite. Phosphoenolpyruvate carboxy kinase, pyruvate
dehydrogenase, succinate dehydrogenase, NADP+-linked malic enzyme,
ADH fumarate reductase, malate dehydrogenase, and α-ketoglutarate dehydrogenase
and glycerol kinase on the other hand had specific activities greater than 60
nanomoles/min/mg protein. These enzyme activities could account for the
production of pyruvate, acetate, succinate and glycerol. The results further
show that the amount of glycerol produced was 35-48% of the combined total of
pyruvate, acetate and succinate produced. It is apparent that
some of the glycerol 3-phosphate produced in  glycolysis in the presence of salicylhydroxamic
acid is dephosphorylated to form glycerol while the rest is oxidised via
cytochrome aa3, to  form acetate, succinate
and pyruvate.
 
Date 2012-12-31T19:58:10Z
2012-12-31T19:58:10Z
1999-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15466
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(5) [October 1999]