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Significance of charge on lysine residue of ovine luteinizing hormone on immunological and biological properties of the hormone

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Title Significance of charge on lysine residue of ovine luteinizing hormone on immunological and biological properties of the hormone
 
Creator Singh, Vinod
Singh, Ranjit C
Dubey, Rajesh K
 
Description 398-404
In order to understand the significance of positive
charge of lysine residues of ovine luteinizing hormone (oLH) on immunological
and biological activity, the ε-NH2 group(s) of ovine LH were
sequentially modified with 2-iminthiolane (2IT) that preserves the positive
charge of the lysine while the overall charge of the hormone remains unchanged.
These studies have also been compared with the oLH modified by N-succinimidyl 3-(2
pyridyldithio) propionate (SPDP) and succinimidyl 6-[3-(2-pyridyldithio)propionamido]
hexanoate (LC-SPDP) that abolish positive charge of lysine residues. The modification
primarily occurs in the α-subunit. Sequential modification led to progressive
reduction in receptor binding and immunological activities. However, the
steroidogenic activity was substantially retained. The immunoreactivity and receptor
binding properties of 2IT modified oLH (oLH-2 IT) were less affected when compared
to SPDP (oLH-SPDP) or LC-SPDP (oLH-LC-SPDP) modified derivatives suggesting that
increase in hydrophobic carbon chain in oLH -LC-SPDP molecule resulted in drastic
inhibition in immunological and biological properties. But the steroidogenic
potentia1 of oLH -2IT, oLH- LC- SPDP or oLH-SPDP was relatively comparable.
This suggests that a single -NH2 group modification with 2IT would generate
the site in the hormone for conjugation to the toxin/carrier proteins that may
retain better immunological and biological activity compared to that of SPDP or
LC-SPDP modified oLH.
 
Date 2012-12-31T20:04:36Z
2012-12-31T20:04:36Z
1999-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15469
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(6) [December 1999]