ICAR Research Data Repository for Knowledge Management
Cloning, expression and purification of the DNA binding domain of RFX protein
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Cloning, expression and purification of the DNA binding domain of RFX protein
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| Creator |
Panchal, S C
Madan, Anup Hosur, R V |
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| Description |
429-432
The RFX DNA binding domain (DBD) is a novel highly conserved motif belonging to a large number of dimer DNA binding proteins which have diverse regulatory functions in eukaryotic organisms. To characterize this novel motif, a 78mer polypeptide corresponding to the DBD of human hRFX (hrfX1/DBD), a prototypical member of the RFX family has been cloned and overproduced in Escherichia coli. A purification procedure using cation exchange chromatography has also been developed. |
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| Date |
2012-12-31T20:05:09Z
2012-12-31T20:05:09Z 1999-12 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15470 |
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| Language |
en_US
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| Rights |
 CC Attribution-Noncommercial-No Derivative Works 2.5 India
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBB Vol.36(6) [December 1999]
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