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Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass

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Title Immobilization of Amaranthus leaf oxalate oxidase on alkylamine glass
 
Creator Pundir, C S
Goyal, L
Thakur, M
Bhargava, A K
 
Description 449-452
A membrane bound oxalate oxidase from leaves of Amaranthus -

 spionsus has been partially purified and immobilized on
alkylamine glass with a yield of 9.2 mg protein/g support. The enzyme retained 99.4%
of initial activity of free enzyme after immobilization. There was no change in
the optimum pH (3 .5) and Vmax
but the temperature for maximum activity was slightly decreased (35 °C) and energy of activation (Ea,) and Km for oxalate were increased after
immobilization. The immobilized enzyme preparation was stable for 6 months,
when stored in distilled water

at 4°C. Presence of CI did not affect the activity of
immobilized enzyme.
 
Date 2012-12-31T20:05:45Z
2012-12-31T20:05:45Z
1999-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15471
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.36(6) [December 1999]