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<i style="mso-bidi-font-style: normal">GbWRKY1</i>, a novel cotton (<i style="mso-bidi-font-style: normal">Gossypium barbadense</i>) WRKY gene isolated from a bacteriophage full-length cDNA library, is induced by infection with <i style="mso-bidi-font-style:normal">Verticillium dahliae</i>

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Title GbWRKY1, a novel cotton (Gossypium barbadense) WRKY gene isolated from a bacteriophage full-length cDNA library, is induced by infection with Verticillium dahliae
 
Creator Shu-Ling, Zhang
Xing-Fen, Wang
Yan, Zhang
Jian-Feng, Liu
Li-Zhu, Wu
Dong-Mei, Zhang
Zhi-Ying, Ma
 
Subject cDNA-Expression library
Gossypium barbadense
Verticillium wilt resistance
WRKY gene
 
Description 405-413
WRKY transcription factor proteins play
important roles in diverse stress responses. In this study, we first cloned a
novel WRKY from our constructed bacteriophage
full-length cDNA library for cotton (Gossypium
barbadense). The plants were stressed by exposure to a defoliating strain
of Verticillium dahliae. The capacity
of primary cDNA library was 1.28 × 106 PFU and the titer of the
amplified cDNA library was >1010 PFU mL–1. The recombination
rate of the library was 94% and average insert size was about 1.1 kb. This
novel gene, named GbWRKY1 was 1971 bp
long and encodes a protein of 489 amino acids. It contains two characteristic
WRKY domains and two zinc finger motifs. The sub-cellular assay indicated that GbWRKY1–GFP fusion protein was localized in the nucleus. Furthermore, Northern blot
analysis showed that expression pattern of GbWRKY1
was similar among tissue types (roots, stems and leaves), but differed
between pathogen-infiltrated and Czapek medium-infiltrated (untreated control)
plants. Quantitative real-time PCR showed that GbWRKY1 could also be induced by salicylic acid (SA), methyl
jasmonate (MeJA) and 1-aminocyclopropane-1-carboxylic acid (ACC). These findings clearly suggest that as a pathogen-inducible transcription factor GbWRKY1 plays an important role in plant
defense responses.
 
Date 2012-12-13T07:23:19Z
2012-12-13T07:23:19Z
2012-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15239
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.49(6) [December 2012]