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<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Probing the evolutionary conserved regions within functional site of drug-resistant target proteins of<i style="mso-bidi-font-style:normal"> Staphylococcus aureus: In silico</i> phylogenetic motif profiling approach</span>

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Title Probing the evolutionary conserved regions within functional site of drug-resistant target proteins of Staphylococcus aureus: In silico phylogenetic motif profiling approach
 
Creator Kahlon, Amandeep Kaur
Darokar, Mahendra P
Sharma, Ashok
 
Subject Multi-drug resistance
Staphylococcus aureus
Domain
Co-target
Phylogenetic similarity threshold
Phylogenetic motifs
Interactome analysis
Drug-resistant genes
Conserved residues
Functional partners
 
Description 442-450
Staphylococcus
aureus is one of the major causes of clinical
infections and increasing mortality due to multi-drug resistance. In this
study, eight drug-resistant genes, beta-lactamase, metallo-beta-lactamase,
vanB, mecA, norA, qacA, qacB and qacC of S.
aureus strain Mu50 (vancomycin resistant) were studied to predict the
evolutionary conserved functional site residues in their protein sequences. It
was found that in beta-lactamase, Tyr, Gly, Thr, Asn and in
metallo-beta-lactamase, Thr, His, Gly, Leu, Arg and Asp residues were highly
conserved. In vanB, Gly, His and Asp residues were highly conserved. Whereas in
mecA, His, Val, Phe, Gln, Lys and in norA, Ser, Leu and Ala residues showed conservedness at
moderate level. In the multi-drug efflux pump (corresponding to qacA, qacB and
qacC), Gly residue was found to be highly conserved. The homology clustering of
target proteins through SCI-PHY algorithm and homologues identified through
PSI-BLAST were compared to identify the degree of conservation of functional
residues. The phylogenetic motifs identified using homologues of target
proteins were validated through domain search to locate their site and
functionality in the protein sequences. Interactome analysis was performed to
understand the possible mode of interaction of target proteins with their
functional partners.
 
Date 2012-12-13T07:25:29Z
2012-12-13T07:25:29Z
2012-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/15241
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.49(6) [December 2012]