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<span style="font-size:11.0pt;mso-bidi-font-size: 10.0pt;font-family:"Times New Roman";mso-fareast-font-family:"Times New Roman"; mso-ansi-language:EN-GB;mso-fareast-language:EN-US;mso-bidi-language:AR-SA" lang="EN-GB">Inhibition of dextransucrase activity in <i>Streptococcus mutans</i> by plant phenolics</span>

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Title Inhibition of dextransucrase activity in Streptococcus mutans by plant phenolics
 
Creator Goyal, Dimple
Sharma, Sukesh
Mahmood, Akhtar
 
Subject Dental caries
Polyphenols
Dextransucrase
Streptococcus mutans
Plant chewing sticks
 
Description 48-53
Streptococcus
mutans is responsible for causing dental caries in
humans and utilizes sucrose for its growth. The dextransucrase (EC 2.4.1.5) is
responsible for sucrose metabolism, which exhibits both hydrolytic and
glucosyltransferase activities. In this study, we examined the effects of the
plant phenols, namely gallic, tannic and syringic acids and aqueous extracts of
certain traditionally used chewing sticks (Acacia arabica, Azadirachta
indica, Pongamia pinnata and Salvadora persica) for prevention of
dental caries on hydrolytic activity of dextransucrsae in S. mutans. Gallic acid (4-5 mM) produced 80-90% inhibition
of the enzyme, while tannic acid (0.2 mM) and syringic acid (5 mM) inhibited
the enzyme activity 80% and 48%, respectively in vitro. The aqueous
extracts of chewing sticks produced 35-40% inhibition of dextransucrase
activity at 5 mg phenol concentration. Kinetic analysis revealed mixed-type of
enzyme inhibition by polyphenols, where both Km and Vmax
were altered. The value of Ki
for tannic, gallic and syringic acids were 0.35, 1.6 and 1.94 mM,
respectively. The enzyme inhibition by polyphenols was optimum at pH 7-7.5,
while by plant extract was maximum at pH 5-6. These results suggest that plant
polyphenols may find potential
applications in the prevention and control of dental caries by inhibiting
dextransucrase activity in S. mutans.
 
Date 2013-02-23T11:26:45Z
2013-02-23T11:26:45Z
2013-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/16065
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(1) [February 2013]