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Expression and role of miR-34a in bladder cancer

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Title Expression and role of miR-34a in bladder cancer
 
Creator Wang, Wei
Li, Tan
Han, Guang
Li, Ying
Shi, Li-hua
Li, Hui
 
Subject MicroRNA-34a
Bladder cancer
Pathological variables
Proliferation
Apoptosis
 
Description 87-92
To investigate the biological function of
microRNA-34a (miR-34a) in bladder cancer, the
expression of miR-34a was
determined using quantitative real-time polymerase chain (qRT-PCR) reaction in
42 cases of bladder cancer. The relationship between
the expression of miR-34a
and development of bladder cancer was also studied. The mature mimics of miR-34a were chemically synthesized and
transiently transfected into human bladder cancer T24 cells. The
effects of
miR-34a on apoptosis, cell cycle
and proliferation in T24 cells were evaluated by flow cytometry and MTT,
respectively. The results showed that the low expression rate of miR-34a was correlated with the malignancy and tumor size
of
bladder cancer. The up-regulation of miR-34a in T24 cells contributed to cell growth and cell
cycle arrest, but not
caspase-3 pathway.
These findings suggest that the relative low expression
of miRNA-34a might be
involved in the tumorigenesis of bladder cancer.
 
Date 2013-04-18T07:36:26Z
2013-04-18T07:36:26Z
2013-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/17104
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(2) [April 2013]