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Overexpression of circulating miRNA-21 and miRNA-146a in plasma samples of breast cancer patients

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Title Overexpression of circulating miRNA-21 and miRNA-146a in plasma samples of breast cancer patients
 
Creator Kumar, Subramanian
Keerthana, Ranganathan
Pazhanimuthu, Annamalai
Perumal, Pachiappan
 
Subject Breast cancer
Biomarkers
MicroRNAs
miR-21
miR-146a
Plasma
 
Description 210-214
Breast cancer is the second most common
malignant disease affecting Indian women and is the leading cause of
cancer-related mortality. MicroRNAs (miRNAs) are remarkably stable in blood,
which makes them novel and promising biomarkers for cancer detection and
diagnosis. In this study, we investigated
whether expression levels of circulating specific miRNAs, such as microRNA-21 (miR-21)
and microRNA-146a (miR-146a) could be used as potential biomarkers in plasma of
breast cancer patients and healthy individuals. We compared the expression
levels of breast cancer associated specific miRNAs — miR-21 and miR-146a in
plasma samples of histopathologically reported 14 patients (aged 35-61 yrs)
with breast cancer and an additional 8 healthy volunteers (aged 35-54 yrs). The
miRNA expression level was determined by TaqMan quantitative PCR (qPCR) assay.
The miRNA expression level of each sample was normalized to that of miR-16 as
reference and expressed as relative expression (2-Ct). Our results showed that the circulating level of miR-21 and miR-146a were significantly higher
in plasma samples of breast cancer patients, when compared to those of healthy
controls (p
 
Date 2013-06-04T13:12:39Z
2013-06-04T13:12:39Z
2013-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/18693
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(3) [June 2013]