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Important role of the C-terminal region of pig aldo-keto reductase family 1 member C1 in the NADPH-dependent reduction of steroid hormones

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Title Important role of the C-terminal region of pig aldo-keto reductase family 1 member C1 in the NADPH-dependent reduction of steroid hormones
 
Creator Kwon, Seul Gi
Bang, Woo Young
Jeong, Jin Young
Cho, Hwak Rae
Park, Da Hye
Hwang, Jung Hye
Kim, Tae Wan
Ko, Moon-Suck
Cho, In-Cheol
Joo, Young Kuk
Jeong, Mi-Ae
Kim, Sam Woong
Kim, Chul Wook
 
Subject AKR1C1
NADPH-dependent reduction
Steroid hormone
 
Description 237-241
The NADPH-dependent reduction activities of two
paralogous pig AKR1C1s with and without 19 additional amino acid residues in
C-terminus were evaluated against steroid hormones including
5a-dihydrotestosterone, testosterone, progesterone, androstenedione and 5a-androstane-3,17-dione, which act as substrates of the AKR1C1s. Among the
hormones, the AKR1C1s exhibited the highest activity against 5a-dihydrotestosterone and the lowest activity against testosterone and
progesterone. Furthermore, the AKR1C1s showed the largest differential
activities against
5a-dihydrotestosterone, but no such change of activities was found against
progestrone and testosterone. These results suggest that the C-terminal region
of AKR1C1 plays an important effect in the reduction activities of pig AKR1C1.
Thus, the differential activities of two AKR1C1 paralogs observed in the
present study provide important insights in understanding the molecular
evolution.
 
Date 2013-06-04T13:02:31Z
2013-06-04T13:02:31Z
2013-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/18692
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.50(3) [June 2013]