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Crystallization and structure determination of goat lactoferrin at 4.0 Å resolution: A new form of packing in lactoferrins with a high solvent content in crystals

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Title Crystallization and structure determination of goat lactoferrin at 4.0 Å resolution: A new form of packing in lactoferrins with a high solvent content in crystals
 
Creator Kumar, Pravindra
Yadav, Savita
Singh, Tej P
 
Description 16-21
Lactoferrin was purified from fresh samples
of goat colostrums, saturated with Fe3+ and CO32-
ions and crystallized by microdialysis method. The crystals belong to orthorhombic
space group P212121 with a=104.6 Å, b=153.8Å,
c= 155.1 Å and Z=4. The quality of crystals was poor, thus the intensity data were
restricted to 4.0 Å resolution only. The structure was determined by molecular
replacement method using diferric buffalo lactoferrin as a model. The solution clearly
indicated the presence of one molecule in the asymmetric unit, which
corresponds to a Vm value of 7.1 A3/Da. The structure was refined
with stringent constraints to an R-factor of 0.246 using all the reflections 15,870
to 4.0 Å resolution. The overall structure of goat lactoferrin is essentially similar
to those of buffalo and bovine lactoferrins. However, the iron-binding environment
in goat lactoferrin is somewhat different, in which 2 CO32- ions
have low occupancies. The solvent content of approximately 84% was very high in
the present case which explains the fragility of the crystals of goat lactoferrin.
In a way, it is very surprising that the crystals grow at all, although crystals
with solvent as high as 89% have been reported.
 
Date 2013-07-15T05:44:38Z
2013-07-15T05:44:38Z
2002-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19743
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(1) [February 2002]