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Solubilization of μ-opioid receptors enriched from bovine brain membranes

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Title Solubilization of μ-opioid receptors enriched from bovine brain membranes
 
Creator Sastry, K V H
Yadgiri, B
Reddy, J M
Janardanasarma, M K
 
Description 60-65
Solubilization is the most critical step in
the purification of opioid receptors as these proteins are highly sensitive to detergents
and get inactivated even with very mild detergents. Membranes enriched with μ-opioid
receptors from bovine corpus striatum were solubilized by various methods to obtain
the active soluble receptor suitable for affinity purification.

Solubilization by digitonin resulted in
marginal yields. CHAPS in presence of NaCl could extract active receptor into the
solution. The detergent and NaCl were removed by either polyethylene glycol precipitation
or by desalting on Sephadex G50. The polyethylene glycol precipitation
resulted in the formation of liposomes into which the receptor protein was

incorporated. Liposome formation was not observed
in desalting method and the recovery of the receptor was partial.
 
Date 2013-07-15T06:16:36Z
2013-07-15T06:16:36Z
2002-02
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19749
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(1) [February 2002]