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Purification, characterization and amplification of a 1.8 kbp fragment of xylanase 5 from <i>Aeromonas caviae </i>W-61

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Title Purification, characterization and amplification of a 1.8 kbp fragment of xylanase 5 from Aeromonas caviae W-61
 
Creator Roy, Narayan
Kamio, Yoshiyuki
 
Description 179-184
Aeromonas caviae W-61 produces multiple extracellular
xylanases, the xylanases 1,2,3,4, and 5. In this study, we purified and
characterized the xylanase 5 of A. caviae W-61, and amplified a part of
xylanase 5 gene (xyn5). The purified xylanase 5 was found to be a
single polypeptide with molecular mass of 140 kDa. It was an endo-β-1 ,4-xylanase
showing

optimum temperature 40oC and
optimum pH 6.0. Xylobiose, xylotriose, xylotetrose, xylopentose,
xylohexose and a small amount of xylose were detected as the hydrolysis products.
The N-terminal amino acid sequence and several internal amino acid sequences of
xylanases 5 were determined. From the sequence, a 1.8 kbp fragment was
amplified by PCR using forward and reverse primers. DNA sequencing confirmed the
presence of nucleotide sequences corresponding to the N-terminal amino acid sequence
and the internal amino acid sequences of xylanase 5.
 
Date 2013-07-15T08:51:39Z
2013-07-15T08:51:39Z
2002-06
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19775
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(3) [June 2002]