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Matrix metalloprotease 2-mediated activation of Ca<sup>2+</sup>-ATPase by superoxide radical (O<sub>2</sub><sup>.-</sup>) in plasma membrane of bovine pulmonary vascular smooth muscle

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Title Matrix metalloprotease 2-mediated activation of Ca2+-ATPase by superoxide radical (O2.-) in plasma membrane of bovine pulmonary vascular smooth muscle
 
Creator Mandai, Malay
Das, Sudip
Chakraborti, Tapati
Chakraborti, Sajal
 
Description 390-396
The role of the matrix metalloprotease-2 (MMP-2)
in regulating Ca2+-ATPase activity in bovine pulmonary artery smooth
muscle plasma membranes during treatment with the O2.-generating
system, hypoxanthine (HPX) plus xanthine oxidase (XO) has been studied. The smooth
muscle membranes possess matrix metalloprotease (MMP) activity in gelatin

Zymogram, having an apparent molecular mass
of 72 kDa; the activity is inhibited by the tissue inhibitor of metalloprotease-2
(TIMP-2). Since both protease and MMP-2 have same molecular mass and are inhibited
by TIMP-2, it may, therefore, be suggested that the protease is the MMP-2. Treatment
of the smooth muscle membrane suspension with the O2.- generating
system stimulates MMP-2 activity, as evidenced by an apparent increase in the intensity
of the protease activity. O2.- also enhances [14C]-gelatin
degradation and Ca2+-ATPase activity. The increase in MMP activity, assessed
by [14C] -gelatin degradation and Ca2+-
ATPase activity are inhibited upon pretreatment with superoxide dismutase (SOD).
The O2.- triggered MMP and
Ca2+-ATPase
activities in the membrane are found to be inhibited by TIMP-2 . The stimulation
of the MMP and Ca2+-ATPase activities remain unaffected by the inhibitors
of serine, thiol and cysteine groups of proteases such as phenylmethylsulfonylfluo
ride (PMSF). Bowman Birk inhibitor (BBI), chymostatin, N-ethylmaleimide, leupeptin.
antipain and pepstatin. Adding pure bovine MMP-2 to the smooth muscle membrane suspension
causes an increase in Ca2+-ATPase activity, but the pretreatment with
TIMP-2 inhibits the increase in the enzyme activity.
 
Date 2013-07-15T09:37:48Z
2013-07-15T09:37:48Z
2002-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19783
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(6) [December 2002]