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Purification and characterization of lipoxygenase from aromatic and non-aromatic rice (<i>Oryza sativa </i>L.)

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Title Purification and characterization of lipoxygenase from aromatic and non-aromatic rice (Oryza sativa L.)
 
Creator Basak, Sanjay
Johari, Rajendra Prasad
 
Description 401-405
Lipoxygenase (Lox) has been ex tensively purified
from aromatic (Bas-370) and non-aromatic. (Pusa-834) rice varieties.
Crude isolates of Lox from the aromatic varieties (Bas-370 and PB-1) showed higher
specific activity (4-fold) when compared to non-aromatic varieties (Pusa-677
and Pusa-834). The activity was optimum at pH 8:0 in .all four
varieties.

Anionic PAGE of Lox from three days old
seed lings revealed one extra band (Rm 0.48) III aromatic varieties, besides the
presence of a major band (Rm 0.28) in all the four varieties. Elution profile
of Lox from Bas-370 and Pusa-834 on DEAE-cellulose column showed three distinct
peaks (L-1, L-2 and L-3), L-2 being the major fraction In both the varieties. SDSPAGE
of purified L-2 from Bas-370 showed a single band of molecular mass ~88 kDa.
 
Date 2013-07-15T09:41:22Z
2013-07-15T09:41:22Z
2002-12
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19785
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.39(6) [December 2002]