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Molecular aspects on the interaction of sanguinarine with B-form, Z-form and H<sup>L</sup>- form DNA structures

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Title Molecular aspects on the interaction of sanguinarine with B-form, Z-form and HL- form DNA structures
 
Creator Maiti, M
 
Description 20-26
The interaction of sanguinarine with
right-handed (B-form), left-handed (Z- form) and left-handed (HL-
form) structures of poly(dG-dC).poly(dG-dC) has been investigated by measuring
the circular dichroism (CD) and UV-absorption spectral analysis. Sanguinarine binds
strongly to the B-form DNA and does not bind to Z-form or HL-form,
but it converts the Z form and the HL-form back to the bound right
handed form as evidenced from CD spectroscopy. Sanguinarine inhibits the rate
of B to Z transition under ionic conditions that otherwise favour the
left-handed conformation of the polynucleotides. UV absorption kinetic studies show that the Z-form reverses back to B-form
on binding to sanguinarine. Binding isotherms obtained from spectrophotometric
data show that sanguinarine binds strongly to the B - form polymer in a
non-cooperative manner, in sharp contrast to the highly cooperative interaction
under Z-form and  HL-form
polynucleotides. These studies reveal that the alternating GC sequence undergoes
defined conformation changes and interacts with singuinarine which may be an
important aspect in understanding its extensive biological activities.
 
Date 2013-07-16T05:23:08Z
2013-07-16T05:23:08Z
2001-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19793
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(1-2) [February-April 2001]