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<i>In vitro </i>and <i>in vivo </i>inhibition of telomerase activity from Chinese hamster V79 cells

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Title In vitro and in vivo inhibition of telomerase activity from Chinese hamster V79 cells
 
Creator Pandit, B
Bhattacharyya, NP
 
Description 42-47
While studying the inhibition of
telomerase activity in Chinese hamster V79 cells using polymerase chain
reaction (PCR) based telomeric repeat amplification protocol (TRAP) assay, we
had earlier observed that 7-deaza deoxy guanosine triphosphate (7-deaza dGTP)
and oligonucleotide (TTAGGG)4 inhibited telomerase activity in
vitro. In the present stud y,

we report inhibition of lelomerase
activity by modified base 7-deaza deoxy adenosine triphosphate (7-deaza dATP)
and phosphorothioate TTAGGG (PS-TTAGGG). Both the compounds inhibited
telomerase activity in a concentration dependent manner; 8.5μM of 7-deaza dATP and 0.1μM of PS-TT AGGG being the concentration for 50% of
the maximum inhibition. This observation support s our earlier hypothesis that
incorporation of a modified nucleotide into telomere possibly interferes with
the recognition of the telomerase and TT AGGG interferes with the RNA component
of telomerase. We have further shown that treatment of cells with nicotinamide
(NA) and benzamide (BA), well known inhibitors of poly (ADP-ribose) polymerase,
reduced telomerase activity. We speculate that modification of the telomeric
binding proteins or other components by poly (ADP-ribosyl)ation may be involved
in such inhibition.
 
Date 2013-07-16T05:42:41Z
2013-07-16T05:42:41Z
2001-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19796
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(1-2) [February-April 2001]