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Effect of <i>myo</i>-inositol(1,4,5)trisphosphate on the hydrolysis of phytic acid by phytase

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Title Effect of myo-inositol(1,4,5)trisphosphate on the hydrolysis of phytic acid by phytase
 
Creator Padmanabhan, Usha
Dasgupta, Shashiprabha
Biswas, B B
Dasgupta, Dipak
 
Description 53-55
Phytase is a monomeric enzyme of
molecular mass 160 kDa which catalyzes the hydrolysis of phytic acid (D-myo inositol
hexakisphosphate, InsP6) in a stepwise manner to myo-inositol.
The enzyme-InsPn (n = 1- 6) interaction at the catalytic
site has a dissociation constant in the micro molar range. There also exists in
the enzyme, a non-catalytic site specific for insP3 with
dissociation constant in the nano molar range. We have probed the effect of the
high affinity InsP3 binding on the dissociation constant (Kd)
of the phytase-InsP6 interaction and the kinetics of hydrolysis.
These studies demonstrate the effect exerted by the high affinity InsP3
binding on the catalytic site of the enzyme.
 
Date 2013-07-16T05:46:35Z
2013-07-16T05:46:35Z
2001-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19798
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(1-2) [February-April 2001]