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Interaction of mithramycin with chromatin

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Title Interaction of mithramycin with chromatin
 
Creator Mir, Mohd. Ayoub
Dasgupta, Dipak
 
Description 71-74
Mithramycin (MTR) is an anti-cancer
antibiotic that blocks the macromolecular biosynthesis via reversible
interaction with DNA template in the presence of bivalent metal ion such as Mg2+.
In absence of DNA, mithramycin forms two types of complexes with Mg2+,
complex I (with 1:1 stoichiometry in terms of MTR:Mg2+) and complex
II (with 1:2 stoichiomelry in terms of MTR : Mg2+) . In an
eukaryotic system, the drug would interact with chromatin, a protein - DNA
complex. We have employed the spectroscopic techniques such as absorpt ion and
fluorescence to study the interaction of MTR : Mg2+ complexes with
rat liver chromatin. In this report, we have shown that the two types of
ligands have different binding potentials with the same chromatin. This
supports our proposition that complexes I and II, are different molecular species.
We have also shown that the histone protein (s) reduce the binding potential
and the number of available sites for both ligands.
 
Date 2013-07-16T06:06:57Z
2013-07-16T06:06:57Z
2001-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19801
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.38(1-2) [February-April 2001]