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Actin binds to the cytoplasmic tail of α<sub>1</sub> subunit of integrin in hepatocytes
NOPR - NISCAIR Online Periodicals Repository
View Archive Info| Field | Value | |
| Title |
Actin binds to the cytoplasmic tail of α1 subunit of integrin in hepatocytes
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| Creator |
Menon, Bindu
Sudhakaran, P R |
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| Description |
81-85
α1β1-Integrin is a common receptor for laminin and collagen IV on hepatocytes. The interactions of intracellular domain of integrins with cytoplasmic elements are critical in the initiation and transduction of signals. In order to understand the nature of cytoplasmic components that can interact with cytoplasmic domain of α1 integrin, cytoplasmic extracts of mono layers of rat hepatocytes were subjected to chromatography over an affinity column prepared by coupling a 60-mer synthetic cytoplasmic tai l of α1 subunit. SDS-PAGE analysis of the eluate showed the presence of a 47 kDa protein. Dot-Blot assay using radio- iodinated 47 kDa protein showed the binding of the protein to 60-mer C tail in a concentration dependent manner. Immunoblot analysis using specific antibodies showed that the 47 kDa protein is actin. |
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| Date |
2013-07-16T07:02:55Z
2013-07-16T07:02:55Z 2000-04 |
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| Type |
Article
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| Identifier |
0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19814 |
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| Language |
en_US
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| Rights |
CC Attribution-Noncommercial-No Derivative Works 2.5 India
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| Publisher |
NISCAIR-CSIR, India
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| Source |
IJBB Vol.37(2) [April 2000]
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