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Purification and characterization of an organ specific haemorrhagic toxin from <i>Vipera russelli russelli </i>(Russell's viper) venom

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Title Purification and characterization of an organ specific haemorrhagic toxin from Vipera russelli russelli (Russell's viper) venom
 
Creator Kole, Labanyamoy
Chakrabarty, Dibakar
Datta, Kausiki
Bhattacharyya, Debasish
 
Description 114-120
A haemorrhagic toxin (VRR-12) from Vipera
russelli russelli (Russell's viper) venom has been purified by ionexchange chromatography
on CM-Sephadex C-50 followed by size-exclusion HPLC to electrophoretically homogeneous
state. It is a 12 kDa single polypeptide having 1 mole of  Zn+2 ion. This toxin induces intense
intestinal haemorrhage and to a lesser extent skeletal muscle haemorrhage in mice.
It does not show detectable proteolytic and esterolytic activity with selected
substrates under specified conditions, haemolytic and phospholipase activity. When
VRR-12, preincubated with bivalent anti serum against Saw-scaled and Russell's
viper venom or EDTA was injected, haemorrhagic activity was not reduced, on the
otherhand preincubation with phenylmethyl sulphonyl fluoride reduced the activity
markedly. Biodistribution studies with 1251 VRR- 12 show that haemorrhagic manifestation
by this toxin is not a direct function of the fraction of the totally administered
toxin distributed to that tissue.
 
Date 2013-07-16T07:15:13Z
2013-07-16T07:15:13Z
2000-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19818
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(2) [April 2000]