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Calcium-mediated conversion of sucrose to starch in relation to the activities of amylases and sucrose-metabolizing enzymes in sorghum grains raised through liquid culture

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Title Calcium-mediated conversion of sucrose to starch in relation to the activities of amylases and sucrose-metabolizing enzymes in sorghum grains raised through liquid culture
 
Creator Bhatia, Surekha
Singh, Rangil
 
Description 135-139
Detached ears of sorghum (Sorghum vulgare)
were cultured in complete liquid medium containing Ca2+(0, 3,10 and
30 mM) and effect of this ion on the conversion of· sucrose to starch
with respect to the activities of amylases, sucrose synthase, sucrose phosphate
synthase and soluble invertases were studied in developing grains. Presence of
3 mM Ca2+ in culture medium enhanced both accumulation of starch
and activity of α-amylase in grain but without having any influence on the
activity of β-amylase. However, with 10 and 30
mM Ca2+, the accumulation of starch and activities of both amylases
decreased and with advancement in culturing period, starch accumulation was further
decreased. Irrespective of its concentration, Ca2+ enhanced the activities
of sucrose synthase (synthesis), sucrose-phosphate synthase, soluble acid invertase
and soluble-neutral invertase. Increase in the concentration of Ca2+
in culture medium was concomitant with an elevation in relative proportion of
sucrose in the grain reflecting a net balance in per cent increase with Ca2+
in the activities of sucrose-synthesizing enzymes over sucrose-hydrolysing
ones. Based on the results. it is suggested that assimilation of Ca2+
by grain is essential for maintaining high activity of α-amylase to generate starch
primers required for the conversion or sucrose to starch during grain filling
in sorghum.
 
Date 2013-07-16T07:20:45Z
2013-07-16T07:20:45Z
2000-04
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19821
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(2) [April 2000]