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Effect of glyphosate on the activity of DAHP synthase isozymes in callus cultures of groundnut (<i>Arachis hypogaea </i>L.) selected <i>in vitro</i>

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Title Effect of glyphosate on the activity of DAHP synthase isozymes in callus cultures of groundnut (Arachis hypogaea L.) selected in vitro
 
Creator Jain, Mukesh
Bhalla-Sarin, Neera
 
Description 235-240
Two isozymes of
3-deoxy-D-arabino-heptulosonate-7-phosphate synthase (DAHP synthase or DS, EC
4.1.2.15) from the callus cultures of Arachis hypogaea L. were resolved
by DEAE cellulose column chromatography and characterized using selective
assays based on divalent cation requirements and regulation by the end-product
amino acids. The total extractable activity of DAHP synthase did not show any
change in the glyphosate sensitive and tolerant cell lines when compared to
5-enolpyruvylshikimate-3-phosphate synthase (EPSP synthase, EC 2.5.1.19), the
known target of inhibition by glyphosate. However, the activities of both, DAHP
synthase and EPSP synthase were inhibited by glyphosate in vitro. The
inhibitory effects of glyphosate on the activities of (a) EPSP synthase and
DAHP synthase, and (b) isozymes of DAHP synthase, have been compared. The data
indicate that the Co2+ dependent isozyme of DAHP synthase is a
putative target for the action of glyphosate in A. hypogaea.
 
Date 2013-07-16T08:40:57Z
2013-07-16T08:40:57Z
2000-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19827
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(4) [August 2000]