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Nutrient associated changes in plasma membrane H<sup>+</sup>-ATPase activity of permeabilized <i>Candida albicans </i>cells

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Title Nutrient associated changes in plasma membrane H+-ATPase activity of permeabilized Candida albicans cells
 
Creator Manzoor, Nikhat
Rashid, Bushra
Amin, M
Khan, Luqman A
 
Description 241-244
Nutrient stimulated H+
extrusion by cells and spheroplasts of Candida albicans and its
underlying mechanism has been investigated. Glucose and glutamic acid
stimulated extrusion by a factor of 6 and 1.4 respectively in cells, but had no
effect on spheroplasts. Proline showed no H+ stimulation in cells,
however, it stimulated spheroplasts 1.5 fold. ATPase activity of
toluene-ethanoi-Triton-X-100 (TET) permeabilized cells decreased by 20%
following exposure to glucose and proline and 30% following exposure to
glutamic acid. Permeabilized spheroplasts of Candida albicans showed
drastic reduction in ATPase activity by 40%, 70% and 85% following exposure to
glucose, proline and glutamic acid respectively. Externally added F-actin fully
restored activity in the case of permeabilized cells and partly with
spheroplasts suggesting that ATPase interacts with other membrane proteins
during nutrient stimulation of cells.
 
Date 2013-07-16T08:43:44Z
2013-07-16T08:43:44Z
2000-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19828
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(4) [August 2000]