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Dye-mediated photodynamic inactivation of <i>Bacillus subtilis </i>cells: Involvement of singlet oxygen and superoxide radicals

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Title Dye-mediated photodynamic inactivation of Bacillus subtilis cells: Involvement of singlet oxygen and superoxide radicals
 
Creator Dube, Alok
Bansal, Harsha
Gupta, Pradeep K
 
Description 245-250
Studies were carried out on the
photodynamic inactivation of Bacillus subtilis cells by He-Ne laser
irradiation in presence of methylene blue and toluidine blue. Electron
paramagnetic resonance (EPR) measurements show decrease in the cell membrane
nuidity due to photodynamic damage of the lipophilic compartments. Study of
cell damage in presence of sodium azide, D2O and glutathione as well
as measurements on singlet oxygen/free radical generation by EPR show that with
toluidine blue, singlet oxygen is the major reactive species leading to cell
membrane damage. No free radical generation was observed in toluidine blue
mediated photodamage. However, with methylene blue our observations suggest
that the photodamage arises due to both singlet oxygen mediated membrane damage
and intracellular damage by superoxide radical.
 
Date 2013-07-16T08:45:16Z
2013-07-16T08:45:16Z
2000-08
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19829
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(4) [August 2000]