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Peanut (<i>Arachis hypogaea</i>)<i> </i>lectin: Use in quantitation of desialylation of glycoproteins

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Title Peanut (Arachis hypogaea) lectin: Use in quantitation of desialylation of glycoproteins
 
Creator Chacko, Balu K
Appukuttan, P S
 
Description 294-298
A high affinity lectin from an easily available
source, peanut (Arachis hypogaea)  agglutinin (PNA) which specifically recognizes
desialylated versions of sialylated oligosaccharides is a unique tool in glycoconjugate
biotechnology. By a single step affinity chromatography on cross- linked guar
galactomannan, PNA was purified to homogeneity with 19 times higher

hemagglutinating activity than the sample
prepared by existing methods involving defatting with organic solvents. Agglutinating
activity of the new preparation remained unchanged for at least 6 months while
PNA prepared from defatted seed lost activity within one week. Glycoproteins desialylated
to varying degrees were prepared by treating bovine fetuin with 0.1 N H2SO4
at 80°C for durations of 10 seconds and above. Enzyme-linked lectin assay of
desialylation of differentially desialylated glycoproteins coated on microplates,
using horse radish peroxidase (HRP) conjugate of PNA (PNA-HRP), along with sialic
content assay revealed that PNA can be used as a quantitative probe for assay of
desialylation in sialylated glycoproteins.
 
Date 2013-07-16T08:59:27Z
2013-07-16T08:59:27Z
2000-10
 
Type Article
 
Identifier 0975-0959 (Online); 0301-1208 (Print)
http://hdl.handle.net/123456789/19835
 
Language en_US
 
Rights CC Attribution-Noncommercial-No Derivative Works 2.5 India
 
Publisher NISCAIR-CSIR, India
 
Source IJBB Vol.37(5) [October 2000]